SDHAF1 Antibody

• Catalog Number: orb20325
• Category: Antibodies
• Description: Goat polyclonal to SDHAF1
• Target: SDHAF1
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: HDSTGAPETRPDGR
• RRID: AB_10753540
• MW: 12.8
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:16000, WB: 0.3-1 μg/ml, IHC-P: 5 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 19kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 12.8kDa according to NP_001036096.1). Recommended concentration: 0.3-1 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti SDHAF1, succinate dehydrogenase complex assem
• Note: For research use only
• Entrez: 644096

orb20325 (0.3ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemical staining of Human Placenta using SDHAF1 antibody