Goat anti-SCRIB / Scribble Antibody

SKU: orb555861

Description

Goat polyclonal antibody to SCRIB

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, IF, WB
• Dilution Range: P-ELISA: 1:1000., IF: 10 μg/ml
• Reactivity: Human
• Application Notes:
  WB: Approx 200-250kDa band observed in lysates of cell lines HeLa, HepG2 and U2OS (calculated MW of 178kDa according to NP_874365.3). This molecular weight is routinely observed by other sources. Recommended concentration: 1-3µg/ml. Primary incubation 1 hour . Notes: Immunofluorescence: Strong expression of the protein seen in the cell junctions of MCF7 cells. Recommended concentration: 10µg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: SCRIB / Scribble
• Protein Sequence: PEGPGKEKELPGQ
• Molecular Weight: 178; 175
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

SCRIB; scribbled homolog (Drosophila); CRIB1; SCRB1; SCRIB1; Vartul; scribble

Quick Database Links

Gene Symbol

SCRIB / Scribble

NCBI Gene ID

23513