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Catalog Number | orb555861 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to SCRIB |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IF, WB |
Reactivity | Human |
Dilution range | P-ELISA: 1:1000., IF: 10 μg/ml |
Conjugation | Unconjugated |
MW | 178; 175 |
Target | SCRIB / Scribble |
Entrez | 23513 |
Protein Sequence | PEGPGKEKELPGQ |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | SCRIB; scribbled homolog (Drosophila); CRIB1; SCRB Read more... |
Note | For research use only |
Application notes | WB: Approx 200-250kDa band observed in lysates of cell lines HeLa, HepG2 and U2OS (calculated MW of 178kDa according to NP_874365.3). This molecular weight is routinely observed by other sources. Recommended concentration: 1-3µg/ml. Primary incubation 1 hour . Notes: Immunofluorescence: Strong expression of the protein seen in the cell junctions of MCF7 cells. Recommended concentration: 10µg/ml. |
Expiration Date | 12 months from date of receipt. |