SCAP2/PRAP Antibody

• Catalog Number: orb18961
• Category: Antibodies
• Description: Goat polyclonal antibody to SKAP2
• Target: SCAP2 / PRAP
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: GLVPKAYIMEMYDI
• RRID: AB_10753005
• MW: 41.2
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:32000, WB: 0.5-2 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 38kDa band observed in lysates of cell line Jurkat (calculated MW of 41.2kDa according to NP_003921.2). In transfected HEK293 transiently expressing SKAP2 a band of approx. 45kDa is observed. This band is not observed in the non-transfected HEK293. The calculated Recommended concentration: 0.5-2 μg/ml. An additional band of unknown identity was also consistently observed in Jurkat at 28kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti SKAP2 antibody, anti SCAP2 antibody, anti PRA
• Note: For research use only
• Entrez: 8935

Western blot analysis of Jurkat lysate using SKAP2 antibody

Western blot analysis of HEK293 lysate using SKAP2 antibody