SCAP2/PRAP Antibody

SKU: orb18961

Description

Goat polyclonal antibody to SKAP2

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: ELISA: 1:32000, WB: 0.5-2 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 38kDa band observed in lysates of cell line Jurkat (calculated MW of 41.2kDa according to NP_003921.2). In transfected HEK293 transiently expressing SKAP2 a band of approx. 45kDa is observed. This band is not observed in the non-transfected HEK293. The calculated Recommended concentration: 0.5-2 μg/ml. An additional band of unknown identity was also consistently observed in Jurkat at 28kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: SCAP2 / PRAP
• Molecular Weight: 41.2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti SKAP2 antibody, anti SCAP2 antibody, anti PRAP antibody, anti src family associated phosphoprotein 2 antibody, anti MGC10411 antibody, anti MGC33304 antibody, anti RA70 antibody, anti SAPS antibody, anti SCAP2 antibody, anti SKAP-HOM antibody, anti SKAP55R antibody, anti Fyn-associated phosphoprotein SKAP55 homologue antibody, anti Pyk2/RAFTK-associated protein antibody, anti src family associated phosphoprotein 2 antibody, anti src kinase-associated phosphoprotein of 55-related protein antibody, anti src-associated adaptor protein antibody

Western blot analysis of Jurkat lysate using SKAP2 antibody

Western blot analysis of HEK293 lysate using SKAP2 antibody

Quick Database Links

Gene Symbol

SCAP2 / PRAP

NCBI Gene ID

8935