SAP130/SF3B3 Antibody

• Catalog Number: orb18410
• Category: Antibodies
• Description: Goat polyclonal antibody to SF3B3
• Target: SAP130 / SF3B3
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: VSKKLEDIRTRYAF
• RRID: AB_10751726
• MW: 136
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:2000, WB: 0.1-0.3 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:2000.WB: Approx 150kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 136kDa according to NP_036558.3). Recommended concentration: 0.1-0.3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti SF3B3 antibody, anti SAP130 antibody, anti sp
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 23450
• Expiration Date: 12 months from date of receipt.

orb18410 staining (0.1ug/ml) of nuclear lysate from HeLa cells (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.