Goat anti-Sall4 (mouse) Antibody

• Catalog Number: orb19503
• Category: Antibodies
• Description: Goat polyclonal antibody to Sall4
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Mouse
• Dilution range: ELISA: 1:32000, WB: 0.1-0.3 μg/ml
• Conjugation: Unconjugated
• MW: 113; 66.2
• Target: Sall4 (mouse)
• Protein Sequence: DQSKKGKGKPQN
• RRID: AB_10749920
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti SALL4 antibody, anti sal-like 4 (Drosophila)
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 70kDa band observed in Rat Spleen and Testis lysates (calculated MW of 66.2kDa according to NP_958797.2). Recommended concentration: 0.1-0.3 μg/ml. Additional bands of unknown identity was also consistently observed at 28+24kDa. These bands were successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

orb19503 (0.1ug/ml) staining of Rat Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.