S100A4/CAPL Antibody

• Catalog Number: orb19362
• Category: Antibodies
• Description: Goat polyclonal antibody to S100A4
• Target: S100A4 / CAPL
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: CNEFFEGFPDKQP
• RRID: AB_10750676
• MW: 11.7
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:128000, WB: 0.05-0.2 μg/ml, IHC-P: 1-2 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.IHC: In paraffin embedded Human Small Intestine shows strong nuclear staining in some cells and weaker cytoplasm staining in some other cells . Recommended concentration, 2-3ug/ml.WB: Approx 13kDa band observed in Human Placenta lysates (calculated MW of 11.7kDa according to NP_002952.1and NP_062427.1). Recommended concentration: 0.03-0.1 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti S100A4 antibody, anti CAPL antibody, anti S10
• Research Area: Cardiovascular Disease, Epigenetics
• Note: For research use only
• Entrez: 6275
• Expiration Date: 12 months from date of receipt.

References

Vogel, Sebastian et al. Activated platelets interfere with recruitment of mesenchymal stem cells to apoptotic cardiac cells via high mobility group box 1/Toll-like receptor 4-mediated down-regulation of hepatocyte growth factor receptor MET J Biol Chem, 289, 11068-11082 (2014)

PubMed: 24567328

Applications

IF

Reactivity

Human

Western blot analysis of Human Placenta lysate using S100A4 antibody

Immunohistochemical of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemical staining of Human Small Intestine using S100A4 antibody