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Item 1 of 2
Goat anti-RXR beta Antibody
Catalog Number: orb18900
| Catalog Number | orb18900 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal antibody to RXRB |
| Target | RXR beta |
| Clonality | Polyclonal |
| Species/Host | Goat |
| Conjugation | Unconjugated |
| Reactivity | Bovine, Canine, Human, Mouse, Porcine, Rat |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Protein Sequence | CRDGMGDSGRDSRSP |
| RRID | AB_10755597 |
| MW | 56.9 |
| Tested applications | ELISA, WB |
| Dilution range | ELISA: 1:128000, WB: 1-3 μg/ml |
| Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 55kDa band observed in lysates of cell line A431 (calculated MW of 56.9kDa according to NP_068811.1). In transfected HEK293 transiently expressing RXRB a band of approx. 60kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3 μg/ml. |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Alternative names | anti RXRB antibody, anti NR2B2 antibody, anti DAUD Read more... |
| Note | For research use only |
| Entrez | 6257 |
orb18900 staining (1ug/ml) of A431 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
HEK293 overexpressing RXRB (RC200516) and probed with orb18900 (mock transfection in first lane), tested by Origene.
