RUTBC3 Antibody

• Catalog Number: orb20290
• Category: Antibodies
• Description: Goat polyclonal antibody to SGSM3
• Target: RUTBC3
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: RDHENYVACSRSHRR
• RRID: AB_10751892
• MW: 85.4
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:4000, WB: 2-3 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx. 85kDa band observed in lysates of Human Lung carcinoma cell line A549 (calculated MW of 85.4kDa according to NP_056520.2). Recommended concentration: 2-3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti SGSM3 antibody, anti small G protein signalin
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 27352

orb20290 (2ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.