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Catalog Number | orb19118 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to RPS19 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Bovine, Canine, Human, Mouse, Rat |
Dilution range | ELISA: 1:16000, WB: 0.3-1 μg/ml, IHC-P: 5 μg/ml |
Conjugation | Unconjugated |
MW | 16.1 |
Target | RPS19 |
Entrez | 6223 |
Protein Sequence | KMVEKDQDGGRK |
RRID | AB_10753320 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti RPS19 antibody, anti ribosomal protein S19 an Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 15kDa band observed in Human lysates of cell lines Daudi and K562 (calculated MW of 16.1kDa according to NP_001013.1). In transfected HEK293 transiently expressing RPS19 a band of approx. 18kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.03-0.1 μg/ml. |
Expiration Date | 12 months from date of receipt. |
orb19118 (0.03ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
HEK293 overexpressing RPS19 (RC205803) and probed with orb19118 (mock transfection in first lane), tested by Origene.
Immunohistochemical staining of Human Cortex using RPS19 antibody
Immunohistochemical staining of Human Liver using RPS19 antibody
Immunohistochemical staining of Human Tonsil using RPS19 antibody