RPE65 Antibody

SKU: orb19749

Description

Goat polyclonal to RPE65

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:32000, WB: 0.5-1.5 μg/ml
• Reactivity: Rat
• Predicted Reactivity: Canine, Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 60kDa band observed in Rat Retina lysates (calculated MW of 60.9kDa according to Human NP_000320.1 and 61.0kDa according to Rat NP_446014.1). Recommended concentration: 0.5-1.5 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: RPE65
• Protein Sequence: EVKKNARKAPQPE
• Molecular Weight: 60.9
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti mRPE65 antibody, anti sRPE65 antibody, anti retinal pigment epithelium-specific protein 65kDa antibody, anti LCA2 antibody, anti RP20 antibody, anti rd12 antibody, anti RBP-binding membrane protein antibody, anti retinal pigment epithelium-specific 61 kDa protein antibody, anti retinal pigment epithelium-specific protein (65kD) antibody, anti retinitis pigmentosa 20 antibody

orb19749 (0.5ug/ml) staining of Rat Retina lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

RPE65

NCBI Gene ID

6121