Goat anti-RPA1 / RPA70 (aa323-337) Antibody

• Catalog Number: orb137106
• Category: Antibodies
• Description: Goat polyclonal to RPA1
• Target: RPA1 / RPA70 (aa323-337)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 0.3-1 μg/ml.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: CKSYEDATKITVRSN
• MW: 68.1
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:128000, WB: 0.3-1 μg/ml
• Application notes: Approx 70+80kDa band observed in lysates of cell line HeLa (calculated MW of 68.1kDa according to NP_002936.1). The upper band likely represents the acetylated protein. Recommended concentration: 0.3-1 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti RPA1 antibody, anti replication protein A1, 7
• Note: For research use only
• Entrez: 6117

Western blot analysis of HeLa lysate (35ug protein in RIPA buffer) using RPA1 antibody

Western blot analysis of NIH3T3 (Lane 1), 3T3L1 (Lane 2), Mouse Lymph Node (Lane 3) and NSO (Lane 4) lysates using RPA1 antibody