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Catalog Number | orb137106 |
---|---|
Category | Antibodies |
Description | Goat polyclonal to RPA1 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Human |
Concentration | 0.3-1 μg/ml. |
Dilution range | ELISA: 1:128000, WB: 0.3-1 μg/ml |
Conjugation | Unconjugated |
MW | 68.1 |
Target | RPA1 / RPA70 (aa323-337) |
Entrez | 6117 |
Protein Sequence | CKSYEDATKITVRSN |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti RPA1 antibody, anti replication protein A1, 7 Read more... |
Note | For research use only |
Application notes | Approx 70+80kDa band observed in lysates of cell line HeLa (calculated MW of 68.1kDa according to NP_002936.1). The upper band likely represents the acetylated protein. Recommended concentration: 0.3-1 μg/ml. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of HeLa lysate (35ug protein in RIPA buffer) using RPA1 antibody
Western blot analysis of NIH3T3 (Lane 1), 3T3L1 (Lane 2), Mouse Lymph Node (Lane 3) and NSO (Lane 4) lysates using RPA1 antibody