RNF34/RFI (internal) Antibody

SKU: orb19887

Description

Goat polyclonal antibody to RNF34

Images & Validation

• Tested Applications: ELISA
• Dilution range: ELISA: 1:64000, WB: 0.3 μg/ml
• Predicted Reactivity: Bovine, Canine, Human, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Preliminary experiments gave an approx 35kDa band in Human Lymph Node, Ovary and Testis lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 41.7kDa according to NP_919247.1. The 35kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: RNF34 / RFI (internal)
• Molecular Weight: 41.7; 41.6
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti RNF34 antibody, anti ring finger protein 34 antibody, anti FLJ21786 antibody, anti RFI antibody, anti RIF antibody, anti RIFF antibody, anti FYVE-RING finger protein MOMO antibody, anti RING finger protein RIFF antibody, anti hRFI antibody

Quick Database Links

Gene Symbol

RNF34 / RFI (internal)

NCBI Gene ID

80196