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Description
Images & Validation
−| Tested Applications | ELISA, FC, IF |
|---|---|
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:2000. Immunofluorescence: Strong expression of the protein seen in the nuclei of HepG2 cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml. |
| Reactivity | Human |
| Predicted Reactivity | Bovine, Canine, Mouse |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Target | RNF3 (247 aa isoform) |
| Protein Sequence | PLLLHYRPKMDLL |
| Molecular Weight | 28.1 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−anti RNF3 antibody, anti DONG1 antibody, anti RNF3A antibody, anti MGC40413 antibody, anti ring finger protein 3 antibody, anti PCGF3 antibody, anti polycomb group ring finger 3 antibody, anti DKFZp686D20235 antibody, anti FLJ36550 antibody, anti FLJ43813 antibody, anti MGC129615 antibody, anti antibody

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Protocol Information
FC
Flow Cytometry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)

