RNF3 (247 aa isoform) Antibody

SKU: orb18429

Description

Goat polyclonal to RNF3

Images & Validation

• Tested Applications: ELISA, FC, IF
• Dilution Range: Peptide ELISA: antibody detection limit dilution 1:2000. Immunofluorescence: Strong expression of the protein seen in the nuclei of HepG2 cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: RNF3 (247 aa isoform)
• Protein Sequence: PLLLHYRPKMDLL
• Molecular Weight: 28.1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti RNF3 antibody, anti DONG1 antibody, anti RNF3A antibody, anti MGC40413 antibody, anti ring finger protein 3 antibody, anti PCGF3 antibody, anti polycomb group ring finger 3 antibody, anti DKFZp686D20235 antibody, anti FLJ36550 antibody, anti FLJ43813 antibody, anti MGC129615 antibody, anti antibody

Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Quick Database Links

Gene Symbol

RNF3 (247 aa isoform)

NCBI Gene ID

10336