Goat anti-RNF213 / C17orf27 (C Terminus) Antibody

SKU: orb19366

Description

Goat polyclonal antibody to RNF213

Research Area

Cancer, Epigenetics

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution Range: ELISA: 1:4000, IHC-P: 2.5µg/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.IHC: In paraffin embedded human kidney shows rare staining of nucleoli across the kidney . Recommended concentration, 2.5ug/ml.WB: Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the calculated size of 374kDa according to NP_065965.3. Therefore we cannot recommend an optimal concentration and the product is investigative grade. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: RNF213 / C17orf27 (C Terminus)
• Protein Sequence: KTAAVLKWNRE
• Molecular Weight: 596
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti RNF213 antibody, anti C17orf27 antibody, anti ring finger protein 213 antibody, anti chromosome 17 open reading frame 27 antibody, anti KIAA1554 antibody

orb19366 (2.5ug/ml) staining of paraffin embedded human kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining

Quick Database Links

Gene Symbol

RNF213 / C17orf27 (C Terminus)

NCBI Gene ID

57674