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RNASEN/Drosha Antibody

SKU: orb19373

Description

Goat polyclonal to RNASEN

Images & Validation

Tested ApplicationsELISA, WB
Dilution rangeELISA: 1:16000, WB: 0.3-1 μg/ml
ReactivityHuman
Predicted ReactivityCanine, Mouse, Rat
Application Notes
This antibody also cross-reacts with human Ribonuclease III (GeneID 29102; NP_037367.2).ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 150kDa band observed in Human Liver lysates (calculated MW of 151kDa according to NP_081075.2 and 159kDa according to Human NP_037367.2). Recommended concentration: 0.5-1.5 μg/ml.

Key Properties

HostGoat
ClonalityPolyclonal
TargetRNASEN / Drosha
Molecular Weight151
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
DisclaimerFor research use only

Alternative Names

anti Rnasen antibody, anti Drosha antibody, anti ribonuclease III, nuclear antibody, anti 1110013A17Rik antibody, anti AI874853 antibody, anti Etohi2 antibody, anti MGC115770 antibody, anti Rn3 antibody, anti ethanol induced 2 antibody, anti ribonuclease III antibody

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RNASEN/Drosha Antibody

orb19373 (0.5ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

RNASEN/Drosha Antibody

Western blot analysis of Human Liver lysate (35ug protein in RIPA buffer) using RNASEN antibody

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

RNASEN/Drosha Antibody (orb19373)

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