RNASEN/Drosha Antibody

• Catalog Number: orb19373
• Category: Antibodies
• Description: Goat polyclonal to RNASEN
• Target: RNASEN / Drosha
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: KQTDKQKLAQRE
• RRID: AB_10753328
• MW: 151
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:16000, WB: 0.3-1 μg/ml
• Application notes: This antibody also cross-reacts with human Ribonuclease III (GeneID 29102; NP_037367.2).ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 150kDa band observed in Human Liver lysates (calculated MW of 151kDa according to NP_081075.2 and 159kDa according to Human NP_037367.2). Recommended concentration: 0.5-1.5 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti Rnasen antibody, anti Drosha antibody, anti r
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 29102

orb19373 (0.5ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot analysis of Human Liver lysate (35ug protein in RIPA buffer) using RNASEN antibody