Ribosomal protein L22 Antibody

SKU: orb19675

Description

Goat polyclonal antibody to RPL22

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution range: ELISA: 1:16000, WB: 0.5 μg/ml, IHC-P: 3.75 μg/ml
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave bands at approx 17-18kDa and 25kDa in Human Liver lysates after 0.5 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 14.8kDa according to NP_000974.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Ribosomal protein L22
• Molecular Weight: 14.8
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 3-6 μg/ml
• Disclaimer: For research use only

Alternative Names

anti RPL22 antibody, anti ribosomal protein L22 antibody, anti EAP antibody, anti HBP15 antibody, anti HBP15/L22 antibody, anti 60S ribosomal protein L22 antibody, anti EBER-associated protein antibody, anti Epstein-Barr virus small RNA-associated protein antibody, anti Epstein-Barr-encoded RNA-associated protein antibody, anti heparin-binding protein 15 antibody, anti heparin-binding protein HBp15 antibody

Immunohistochemical staining of Human Cortex using RPL22 antibody

Immunohistochemical staining of Human Thyroid using RPL22 antibody

Immunohistochemical staining of Human Testis using RPL22 antibody

Immunohistochemical staining of Human Uterus using RPL22 antibody

Quick Database Links

Gene Symbol

Ribosomal protein L22

NCBI Gene ID

6146