Rhophilin 2 (Internal) Antibody

SKU: orb19082

Description

Goat polyclonal antibody to RHPN2

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution range: ELISA: 1:32000, WB: 0.3 μg/ml, IHC-P: 5-10 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx 35kDa band in Human Pancreas and Prostate lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 77kDa according to NP_149094.2. The 35kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Rhophilin 2 (Internal)
• Molecular Weight: 77
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti RHPN2 antibody, anti p76RBE antibody, anti rhophilin, Rho GTPase binding protein 2 antibody, anti rhophilin 2 antibody, anti rhophilin-2 antibody, anti RhoB effector antibody, anti rhophilin-like protein antibody, anti P76RBE antibody, anti RHOBP antibody, anti 76 kDa RhoB effector protein antibody, anti GTP-Rho-binding protein 2 antibody, anti rhophilin-like Rho-GTPase binding protein antibody

Immunohistochemical staining of Human Kidney using RHPN2 antibody

Quick Database Links

Gene Symbol

Rhophilin 2 (Internal)

NCBI Gene ID

85415