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Renalase (aa 224 to 233) Antibody

SKU: orb18989

Description

Goat polyclonal antibody to RNLS

Research Area

Cardiovascular Disease

Images & Validation

Tested ApplicationsELISA, WB
Dilution RangeELISA: 1:128000, WB: 1-3 μg/ml
ReactivityHuman, Mouse, Rat
Predicted ReactivityBovine, Canine
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.IHC: In paraffin embedded Human Kidney shows vesicular staining in the epithelial cells of the CT. Data obtained from a previous batch (different goat). Recommended concentration, 2-4µg/ml.WB: Approx 38+35kDa bands observed in Human Heart lysates, 37kDa band observed in Mouse Heart lysates and approx 35kDa in Rat Heart lysates (calculated MW of 37.8 and 34.9 according to Human NP_001026879.2 and NP_060833.1 resp, of 33.3kDa according to Mouse NP_001161290.1 and of 35.0kDa according to Rat NP_001014189.1). Recommended concentration: 1-3µg/ml.

Key Properties

HostGoat
ClonalityPolyclonal
TargetRenalase (aa 224 to 233)
Protein SequenceVSIDNKKRNI
Molecular Weight37.8; 34.9
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti renalase antibody, anti C10orf59 antibody, anti chromosome 10 open reading frame 59 antibody, anti HGNC:25641 antibody, anti FLJ11218 antibody, anti hypothetical protein LOC55328 antibody, anti RNLS antibody, anti renalase, FAD-dependent amine oxidase antibody, anti RENALASE antibody
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Renalase (aa 224 to 233) Antibody

Immunohistochemical staining of Human Kidney using RNLS antibody

Renalase (aa 224 to 233) Antibody

Western blot analysis of Human Heart (A), Mouse Heart (B), Rat Heart (C) lysates using RNLS antibody (Antibody)

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Renalase (aa 224 to 233) Antibody (orb18989)

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100 μg
$ 530.00
DispatchUsually dispatched within 5-10 working days
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