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Goat anti-Rat IgG (H&L), ALP conjugated
SKU: orb699953
Description
Images & Validation
−
| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution range | 1 : 500-1 :2000 (ELISA), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB) |
| Reactivity | Rabbit |
| Application Notes |
Key Properties
−| Host | Goat |
|---|---|
| Clonality | Polyclonal |
| Immunogen | Purified Rat IgG, whole molecule |
| Purity | Immunogen affinity purified goat IgG. |
| Purification | ALP conjugate is supplied in 30 mM Triethanolamine, pH 7,2, 5 mM Magnesium Chloride, 0,1 mM Zinc Chloride, 1 % (w/v) BSA, Protease/IgG free, 0,05 % (w/v) of sodium azide is added as preservative |
Storage & Handling
−| Storage | Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming. |
|---|---|
| Form/Appearance | Liquid |
| Disclaimer | For research use only |
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Goat anti-Rat IgG (H&L), ALP conjugated (orb699953)
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