RARA Antibody

SKU: orb18965

Description

Goat polyclonal antibody to RARA

Research Area

Cancer, Epigenetics

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:8000, WB: 0.5-2 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.IHC: In paraffin embedded Human Cerebellum shows staining of Purkinje cytoplasm. Recommended concentration, 4-6ug/ml.WB: Approx 50kDa band observed in Human Brain (Cerebellum and Hippocampus) lysates (calculated MW of 50.8kDa according to NP_000955.1; NP_001019980.1). Recommended concentration: 1-3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: RARA
• Protein Sequence: SPSLSPSSHRSSPATQSP
• Molecular Weight: 50.8; 50.8; 39.7
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti RARA antibody, anti RAR antibody, anti NR1B1 antibody, anti retinoic acid receptor, alpha antibody, anti Retinoic acid receptor, alpha polypeptide antibody, anti nucleophosmin-retinoic acid receptor alpha fusion protein NPM-RAR long form antibody, anti nucleophosmin-retinoic acid receptor alpha fusion protein NPM-RAR short form antibody

orb18965 (0.05ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb18965 (4ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

RARA

NCBI Gene ID

5914