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Catalog Number | orb18965 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to RARA |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Bovine, Canine, Human, Mouse, Rat |
Dilution range | ELISA: 1:8000, WB: 0.5-2 μg/ml |
Conjugation | Unconjugated |
MW | 50.8; 50.8; 39.7 |
Target | RARA |
Entrez | 5914 |
Protein Sequence | SPSLSPSSHRSSPATQSP |
RRID | AB_10749754 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti RARA antibody, anti RAR antibody, anti NR1B1 Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.IHC: In paraffin embedded Human Cerebellum shows staining of Purkinje cytoplasm. Recommended concentration, 4-6ug/ml.WB: Approx 50kDa band observed in Human Brain (Cerebellum and Hippocampus) lysates (calculated MW of 50.8kDa according to NP_000955.1; NP_001019980.1). Recommended concentration: 1-3 μg/ml. |
Expiration Date | 12 months from date of receipt. |
orb18965 (0.05ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
orb18965 (4ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.