RAP80 Antibody

• Catalog Number: orb20320
• Category: Antibodies
• Description: Goat polyclonal antibody to UIMC1
• Target: RAP80
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Porcine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: TRRQKEAKTKSDSG
• RRID: AB_10748375
• MW: 79.7
• Tested applications: ELISA, IHC
• Dilution range: ELISA: 1:32000, WB: 0.3 μg/ml, IHC-P: 3.75 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx 60kDa band in Human Heart and Spleen lysates and in lysates of cell lines A431, HeLa and HepG2 after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 79.7kDa according to NP_057374.3. The 60kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti UIMC1 antibody, anti ubiquitin interaction mo
• Note: For research use only
• Entrez: 51720