RANBP8/IPO8 Antibody

SKU: orb18569

Description

Goat polyclonal antibody to IPO8

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution range: ELISA: 1:32000, WB: 1 μg/ml, IHC-P: 4-6µg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Porcine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx 23kDa band in lysates of cell lines A431, A549 and HeLa after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 120kDa according to NP_006381.2. The 23kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: RANBP8 / IPO8
• Molecular Weight: 120; 96.0
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti IPO8 antibody, anti importin 8 antibody, anti RANBP8 antibody, anti RAN-binding protein 8 antibody, anti FLJ26580 antibody, anti RAN binding protein 8 antibody

Immunohistochemical staining of Human Tonsil using IPO8 antibody

Quick Database Links

Gene Symbol

RANBP8 / IPO8

NCBI Gene ID

10526