Goat anti-Ramp1 (C-Term., mouse) Antibody

• Catalog Number: orb19922
• Category: Antibodies
• Description: Goat polyclonal antibody to Ramp1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human, Mouse, Rat
• Dilution range: ELISA: 1:64000, WB: 0.3-1 μg/ml
• Conjugation: Unconjugated
• MW: 16.9; 11.5
• Target: Ramp1 (C-Term., mouse)
• Entrez: 10267
• Protein Sequence: DYQSKRTEGIV
• RRID: AB_10750245
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti Ramp1 antibody, anti receptor (calcitonin) ac
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Experiments gave bands at approx. 18kDa and 40kDa in Rat Brain lysate after 0.3 μg/ml antibody staining. The appearance of a band with much higher molecular weight corresponds to earlier findings in literature with different antibodies (Hilairet, J Biol Chem. 2001 Nov 9;276(45):42182-90.; PMID: 11535606). This protein has a calculated MW of 16.9kDa according to Mouse NP_058590.1 and 16.8kDa according to Rat NP_113833.1. Recommended concentration: 0.3-1 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb19922 (0.3ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.