RAD9A Antibody

SKU: orb19414

Description

Goat polyclonal antibody to RAD9A

Research Area

Cancer, DNA Damage, Epigenetics

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:32000, WB: 0.3-1 μg/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 70kDa band observed in lysates of cell lines A431 and Daudi (calculated MW of 42.5kDa according to NP_004575.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Volkmer et al, J Biol Chem. 1999 Jan 8;274(2):567-70.; PMID: 9872989). Recommended concentration: 0.3-1 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: RAD9A
• Protein Sequence: QGPSPVLAEDSEGE
• Molecular Weight: 42.5
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti RAD9A antibody, anti RAD9 homolog A (S. pombe) antibody, anti RAD9 antibody, anti RAD9 (S. pombe) homolog antibody, anti RAD9 homolog antibody, anti RAD9 homolog (S. pombe) antibody, anti cell cycle checkpoint control protein antibody

Western blot analysis of A431cell lysate using RAD9A antibody

Quick Database Links

Gene Symbol

RAD9A

NCBI Gene ID

5883