Goat anti-RAD51C Antibody

• Catalog Number: orb18415
• Category: Antibodies
• Description: Goat polyclonal antibody to Rad51
• Target: RAD51C
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: RGKTFRFEMQRDL
• RRID: AB_10750225
• MW: 14.9; 42.2
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:32000, WB: 1-3 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 45kDa band seen in HeLa lysate [Predicted MW of 41kDa according to NP_478123]. Recommended for use at 1-3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti DNA repair protein RAD51 homolog 1 antibody,
• Research Area: Cancer, DNA Damage, Epigenetics
• Note: For research use only
• Entrez: 5889

orb18415 staining (2ug/ml) of HeLa lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.