Goat anti-Rabbit IgG (H&L), F(ab)'2 fragment, HRP conjugated

SKU: orb700366

Description

Goat polyclonal antibody against Goat anti-Rabbit IgG (H&L) conjugated to HRP

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution range: The optimal working dilution should be determined by the investigator
• Reactivity: Rabbit
• Application Notes:
  This antibody reacts with the heavy chains on rabbit IgG and with the light chains on all rabbit immunoglobulins based on immunoelectrophoresis.No reactivity is observed to non-immunoglobulin rabbit serum proteins based on immunoelectrophoresis.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Immunogen: Purified Rabbit IgG (H&L)
• Purity: Immunogen affinity purified goat IgG, F(ab)'2 fragment.
• Purification: Concentration: 1.0 mg/ml (E 1% at 280 nm = 13.0)HRP-conjugate is supplied in 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 % (w/v) BSA, Protease/IgG free0.1 % (v/v) of Kathon CG is used as preservative. Use of sodium azide will inhibit enzyme activity of horseradish peroxidasePurity is ≥ 90% based on SDS-PAGE. May contain small amounts of intact IgG.

Storage & Handling

• Storage: Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.
• Form/Appearance: Lyophilized
• Disclaimer: For research use only