Goat anti-Pyruvate dehydrogenase / PDHX Antibody

• Catalog Number: orb18997
• Category: Antibodies
• Description: Goat polyclonal antibody to PDHX
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Canine, Human, Mouse, Rat
• Dilution range: ELISA: 1:32000, WB: 0.1-1 μg/ml
• Conjugation: Unconjugated
• MW: 54.2; 51.5; 29.9
• Target: Pyruvate dehydrogenase / PDHX
• Entrez: 8050
• Protein Sequence: KSFKANLENPIRLA
• RRID: AB_10753006
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti PDHX antibody, anti pyruvate dehydrogenase co
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Pancreas, shows cytoplasmic staining of acinar cells, islets of Langerhans and peri-pancreatic fat cells. Recommended concentration, 1-3ug/ml.WB: Approx 50kDa band observed in Human Pancreas lysate (calculated MW of 54.2kDa according to NP_003468.1). In transfected HEK293 transiently expressing PDHX a band of approx. 50kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-1 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb18997 (1ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.