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Item 1 of 4
Item 1 of 4
Goat anti-Pycard (rodent) Antibody
Catalog Number: orb107673
| Catalog Number | orb107673 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal to Pycard |
| Species/Host | Goat |
| Clonality | Polyclonal |
| Tested applications | ELISA, IF |
| Reactivity | Human, Mouse, Rat |
| Dilution range | Peptide-ELISA: 1:32000, IF: 10) μg/ml |
| Purity | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide |
| Conjugation | Unconjugated |
| MW | 21.5 |
| Target | Pycard (rodent) |
| Entrez | 29108 |
| Protein Sequence | DLTDKLVSYYLES |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Alternative names | anti Pycard antibody Read more... |
| Note | For research use only |
| Application notes | Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm of NIH3T3, KNRK and HeLa cells. Recommended concentration: 10µg/ml. |
| Expiration Date | 12 months from date of receipt. |
orb107673 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). egative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
orb107673 Immunofluorescence analysis of paraformaldehyde fixed KNRK cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Egative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
orb107673 Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). egative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Western blot analysis of Mouse (A) and Rat (B) Spleen lysates (35ug protein in RIPA buffer) using Pycard antibody
