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PTPRM Antibody

SKU: orb19714

Description

Goat polyclonal antibody to PTPRM

Images & Validation

Tested ApplicationsELISA, IHC
Dilution rangeELISA: 1:16000, WB: 0.1 μg/ml, IHC-P: 3.75 μg/ml
ReactivityHuman
Predicted ReactivityCanine, Mouse
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave bands at approx 110kDa and 16kDa in Rat Retina lysates after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 165kDa according to Human NP_001098714.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

HostGoat
ClonalityPolyclonal
TargetPTPRM
Molecular Weight165; 164
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
DisclaimerFor research use only

Alternative Names

anti PTPRM antibody, anti protein tyrosine phosphatase, receptor type, M antibody, anti MGC166994 antibody, anti PTPRL1 antibody, anti R-PTP-MU antibody, anti RPTPM antibody, anti RPTPU antibody, anti hR-PTPu antibody, anti protein tyrosine phosphatase mu antibody, anti protein tyrosine phosphatase, receptor type, mu polypeptide antibody

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NCBI Gene Details

No NCBI Gene data available

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Protocol Information

IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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PTPRM Antibody (orb19714)

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Available Sizes

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100 μg
$ 500.00
DispatchUsually dispatched within 5-10 working days
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