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Catalog Number | orb19990 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to Prostaglandin E2 receptor |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, WB |
Reactivity | Human |
Dilution range | ELISA: 1:2000, WB: 1-3 μg/ml |
Conjugation | Unconjugated |
MW | 47.3; 44.9; 43.7 |
Target | PTGER3 / EP3 |
Entrez | 5733 |
Protein Sequence | KLLREPCSVQLS |
RRID | AB_10750248 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti-PTGER3 antibody, anti-EP3 antibody, anti-pros Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:2000.WB: Approx 55kDa band observed in Human Adipose, Kidney, Pancreas and Placenta lysates (calculated MW of 47.3kDa according to NP_000948.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Sung et al, , Cancer Res. 2005 Oct 15;65(20):9304-11; PMID: 16230392). Recommended concentration: 1-3 μg/ml. |
Expiration Date | 12 months from date of receipt. |
orb19990 (1ug/ml) staining of Human Adipose lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.