Goat anti-PTGER3 / EP3 Antibody

SKU: orb19990

Description

Goat polyclonal antibody to Prostaglandin E2 receptor

Research Area

Epigenetics, GPCR, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:2000, WB: 1-3 μg/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:2000.WB: Approx 55kDa band observed in Human Adipose, Kidney, Pancreas and Placenta lysates (calculated MW of 47.3kDa according to NP_000948.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Sung et al, , Cancer Res. 2005 Oct 15;65(20):9304-11; PMID: 16230392). Recommended concentration: 1-3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: PTGER3 / EP3
• Protein Sequence: KLLREPCSVQLS
• Molecular Weight: 47.3; 44.9; 43.7
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti-PTGER3 antibody, anti-EP3 antibody, anti-prostaglandin E receptor 3 (subtype EP3) antibody, anti-EP3-I antibody, anti-EP3-II antibody, anti-EP3-III antibody, anti-EP3-IV antibody, anti-EP3e antibody, anti-MGC141828 antibody, anti-MGC141829 antibody, anti-MGC27302 antibody, anti-PGE receptor, anti-EP3 subtype antibody, anti-prostaglandin E receptor 3, anti-subtype EP3 antibody, anti-prostaglandin E2 receptor antibody, anti-prostaglandin receptor (PGE-2) antibody, anti-pr antibody

orb19990 (1ug/ml) staining of Human Adipose lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

PTGER3 / EP3

NCBI Gene ID

5733