PTBP2 Antibody

SKU: orb19658

Description

Goat polyclonal antibody to PTBP2

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, IF, WB
• Dilution Range: ELISA: 1:1000, WB: 2-3μg/ml, IF/ICC: 10μg/ml
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:1000.WB: Preliminary experiments gave an approx 35kDa band in lysates of cell line NIH3T3 after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 57.5kDa according to NP_067013.1. The 35kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: PTBP2
• Protein Sequence: SKKFKGEDKMD
• Molecular Weight: 58.1; 58.6; 59.6; 58.0; 57.6; 57.5
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti PTBP2 antibody, anti polypyrimidine tract binding protein 2 antibody, anti FLJ34897 antibody, anti MIBP antibody, anti PTB antibody, anti PTBLP antibody, anti brPTB antibody, anti nPTB antibody, anti nPTB5 antibody, anti nPTB6 antibody, anti nPTB7 antibody, anti nPTB8 antibody, anti PTB-like antibody, anti neural polypyrimidine tract binding protein antibody, anti splicing regulator antibody

Immunofluorescence analysis of paraformaldehyde fixed A431 cells using PTBP2 antibody.

Western blot analysis of Mouse Testes (A) lysate and nuclear NIH3T3 (B) cell lysate using PTBP2 antibody.

Quick Database Links

Gene Symbol

PTBP2

NCBI Gene ID

58155