PTBP2 Antibody

• Catalog Number: orb19658
• Category: Antibodies
• Description: Goat polyclonal antibody to PTBP2
• Target: PTBP2
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: SKKFKGEDKMD
• RRID: AB_10748155
• MW: 58.1; 58.6; 59.6; 58.0; 57.6; 57.5
• Tested applications: ELISA, IF, WB
• Dilution range: ELISA: 1:1000, WB: 2-3μg/ml, IF/ICC: 10μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:1000.WB: Preliminary experiments gave an approx 35kDa band in lysates of cell line NIH3T3 after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 57.5kDa according to NP_067013.1. The 35kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti PTBP2 antibody, anti polypyrimidine tract bin
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 58155

Immunofluorescence analysis of paraformaldehyde fixed A431 cells using PTBP2 antibody.

Western blot analysis of Mouse Testes (A) lysate and nuclear NIH3T3 (B) cell lysate using PTBP2 antibody.