PTBP1/PTB Antibody

• Catalog Number: orb18567
• Category: Antibodies
• Description: Goat polyclonal antibody to PTBP1
• Target: PTBP1 / PTB
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Porcine
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: DGIVPDIAVGTKR
• RRID: AB_10767123
• MW: 59.6, 59.0, 57.2; 21.9
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:32000, WB: 0.01-0.3 μg/ml, IHC-P: 2-4 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.IHC: In paraffin embedded Human Skin shows nuclear staining of keratinocytes. Recommended concentration, 5-10ug/ml.WB: Approx 55-60 kDa band observed in lysates of cell line Jurkat (calculated MW of 57.2kDa according to NP_114368.1). Recommended concentration: 1-3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti PTBP1 antibody, anti PTB antibody, anti polyp
• Research Area: Epigenetics
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of staining of HeLa (A), HepG2 (B), Jurkat (C) and HEK293 (D) nuclear lysates (35µg protein in RIPA buffer) using PTBP1 antibody.

Western blot analysis of staining of Human Ovary lysate (35µg protein in RIPA buffer) using PTBP1 antibody.

Immunohistochemical analysis of paraffin embedded Human Kidney. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining with PTBP1 antibody.

Western blot analysis of lysates of cell line NIH3T3 using PTBP1 antibody