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Catalog Number | orb20648 |
---|---|
Category | Antibodies |
Description | Goat polyclonal to PSMB3 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Bovine, Canine, Human, Mouse, Porcine, Rat |
Dilution range | ELISA: 1:16000, WB: 1-3 μg/ml, IHC-P: 10 μg/ml |
Conjugation | Unconjugated |
MW | 22.9 |
Target | PSMB3 |
Entrez | 5691 |
Protein Sequence | NLYELKEGRQ |
RRID | AB_10772228 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti PSMB3 antibody, anti proteasome (prosome, mac Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 24kDa band observed in lysates of cell line K562 (calculated MW of 22.9kDa according to NP_002786.2). Recommended concentration: 1-3 μg/ml. |
Expiration Date | 12 months from date of receipt. |
orb20648 (1ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
orb20648 (10µg/ml) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.