Goat anti-Proenkephalin (mouse) Antibody

SKU: orb19941

Description

Goat polyclonal antibody to PENK

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution Range: ELISA: 1:32000, WB: 0.2 μg/ml, IHC-P: 0.02-0.05µg/ml
• Reactivity: Mouse, Rat
• Application Notes:
  IHC: Frozen section of Rat Brain (Striatum) shows staining of dense enkephalinergic axon plexus. Frozen section of the Mouse Brain (Stria terminalis) shows staining of a dense enkephalinergic fibre network in the bed nucleus. Recommended concentration, 0.02-0.WB: Preliminary experiments gave an approx 40kDa band in Rat Brain lysates after 0.2 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 30.8kDa according to Mouse NP_001002927.1 and 30.9kDa according to Rat NP_058835.1. The 40kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Proenkephalin (mouse)
• Protein Sequence: YKDSSKQDESH
• Molecular Weight: 30.8
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti proenkephalin antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded rat striatum tissue using Proenkephalin antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded mouse stria terminalis tissue using Proenkephalin antibody

Quick Database Links

Gene Symbol

Proenkephalin (mouse)