Goat anti-Proenkephalin (mouse) Antibody

• Catalog Number: orb19941
• Category: Antibodies
• Description: Goat polyclonal antibody to PENK
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC
• Reactivity: Mouse, Rat
• Dilution range: ELISA: 1:32000, WB: 0.2 μg/ml, IHC-P: 0.02-0.05µg/ml
• Conjugation: Unconjugated
• MW: 30.8
• Target: Proenkephalin (mouse)
• Protein Sequence: YKDSSKQDESH
• RRID: AB_10960826
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti proenkephalin antibody
• Note: For research use only
• Application notes: IHC: Frozen section of Rat Brain (Striatum) shows staining of dense enkephalinergic axon plexus. Frozen section of the Mouse Brain (Stria terminalis) shows staining of a dense enkephalinergic fibre network in the bed nucleus. Recommended concentration, 0.02-0.WB: Preliminary experiments gave an approx 40kDa band in Rat Brain lysates after 0.2 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 30.8kDa according to Mouse NP_001002927.1 and 30.9kDa according to Rat NP_058835.1. The 40kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of formalin-fixed paraffin embedded rat striatum tissue using Proenkephalin antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded mouse stria terminalis tissue using Proenkephalin antibody