Prefoldin Antibody

• Catalog Number: orb18833
• Category: Antibodies
• Description: Goat polyclonal to Prefoldin
• Target: Prefoldin
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: DNIREMLMARRAQ
• RRID: AB_10748784
• MW: 14.2
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:32000, WB: 1-3 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments (at 0.2 μg/ml) gave a strong band at approx 20-25kDa band in Human Skeletal Muscle lysates. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 14kDa according to NP_002613. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti PFDN1 antibody, anti PDF antibody, anti PFD1
• Note: For research use only
• Entrez: 5201
• Expiration Date: 12 months from date of receipt.

Western blot analysis of K562 lysate using Prefoldin antibody