PRDM14 (aa414-427) Antibody

• Catalog Number: orb22534
• Category: Antibodies
• Description: Goat polyclonal antibody to PRDM14
• Target: PRDM14 (aa414-427)
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: YRDKHLKYTPCVDK
• RRID: AB_10958043
• MW: 64.1
• Tested applications: ELISA, FC, IF
• Dilution range: ELISA: 1:16000, WB: 0.1-1 μg/ml, IHC-P: 10ug/ml
• Application notes: WB: Preliminary experiments gave an approx 40kDa band in Human, Mouse and Rat Testis lysates after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 64.1kDa according to NP_078780.1. The 40kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti PRDM14 antibody, anti PR domain containing 14
• Research Area: Epigenetics
• Note: For research use only
• Entrez: 63978

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells using PRDM14 antibody.

Flow cytometric analysis of paraformaldehyde fixed HeLa cells using PRDM14 antibody.