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Item 1 of 3
Goat anti-PPP4C Antibody
Catalog Number: orb20241
| Catalog Number | orb20241 |
|---|---|
| Category | Antibodies |
| Description | Goat polyclonal antibody to PPP4C |
| Species/Host | Goat |
| Clonality | Polyclonal |
| Tested applications | ELISA, IHC, WB |
| Reactivity | Canine, Human, Rat |
| Dilution range | ELISA: 1:16000, WB: 0.1-0.3 μg/ml, IHC-P: 2-4µg/ml |
| Conjugation | Unconjugated |
| MW | 35.1 |
| Target | PPP4C |
| Entrez | 5531 |
| Protein Sequence | PQETRGIPSKKP |
| RRID | AB_10749266 |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Alternative names | anti PPP4C antibody, anti protein phosphatase 4 (f Read more... |
| Note | For research use only |
| Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.IHC: In paraffin embedded Human Prostate shows nuclear staining of secretory cells in the gland. Recommended concentration, 2-4ug/ml.WB: Approx 35kDa band observed in HEK293, A431 and HeLa lysates (calculated MW of 35.1kDa according to NP_002711.1). Recommended concentration: 0.1-0.3 μg/ml. |
| Expiration Date | 12 months from date of receipt. |
orb20241 (0.3ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
orb20241 (2.5ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western blot analysis of staining of Rat Spleen (A) and Lung (B) lysate using PPP4C antibody.
