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PPP4C Antibody
SKU: orb20241
Description
Research Area
Epigenetics
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution Range | ELISA: 1:16000, WB: 0.1-0.3 μg/ml, IHC-P: 2-4µg/ml |
| Reactivity | Human, Rat |
| Predicted Reactivity | Canine |
| Application Notes |
Key Properties
−| Host | Goat |
|---|---|
| Clonality | Polyclonal |
| Target | PPP4C |
| Protein Sequence | PQETRGIPSKKP |
| Molecular Weight | 35.1 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−anti PPP4C antibody, anti protein phosphatase 4 (formerly X), catalytic subunit antibody, anti PP4 antibody, anti PPH3 antibody, anti PPX antibody, anti Protein phosphatase 4, catalytic subunit antibody
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orb20241 (0.3ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
orb20241 (2.5ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western blot analysis of staining of Rat Spleen (A) and Lung (B) lysate using PPP4C antibody.
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
PPP4C Antibody (orb20241)
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