PPP2R5E Antibody

SKU: orb18322

Description

Goat polyclonal antibody to PPP2R5E

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:16000, WB: 0.1-0.3 μg/ml
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 55kDa and 40kDa bands seen in mouse brain extracts. Recommended for use at 0.5-2 μg/ml. Please note that the two bands may correspond to two different isoforms. Two isoforms of the epsilon mRNA have been detected in Northern blots (J Biol Chem 1996 Sep 6;271(36):22081-9).

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: PPP2R5E
• Protein Sequence: LKRGLRRDGIIPT
• Molecular Weight: 54.7
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti PPP2R5E antibody, anti protein phosphatase 2, regulatory subunit B (B56), epsilon isoform antibody, anti PP2A, B subunit, B\1 epsilon isoform antibody, anti PP2A, B subunit, R5 epsilon isoform antibody, anti PP2A, B subunit, B56 epsilon isoform antibody, anti PP2A, B subunit, PR61 epsilon isoform antibody, anti Serine/threonine protein phosphatase 2A, 56 kDa regulatory subunit, epsilon isoform antibody, anti protein phosphatase 2, regulatory subunit B\1, epsilon isoform antibody, anti PP2A, B subunit, R5 epsilon antibody, anti epsilon isoform of regulatory subunit B56, protein phosphatase 2A antibody, anti serine/threonine protein phosphatase 2A, 56 kDa regulatory subunit, epsilon antibody

orb18322 staining (0.5ug/ml) of mouse brain extracts (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Western blot analysis of Human Cerebellum (Lane1), Mouse (Lane2) and Rat (Lane3) Brain lysate using PPP2R5E antibody

Quick Database Links

Gene Symbol

PPP2R5E

NCBI Gene ID

5529