Goat anti-PPP2R5E Antibody

• Catalog Number: orb18322
• Category: Antibodies
• Description: Goat polyclonal antibody to PPP2R5E
• Target: PPP2R5E
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Bovine, Canine, Human, Mouse, Rat
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: LKRGLRRDGIIPT
• RRID: AB_10748732
• MW: 54.7
• Tested applications: ELISA, WB
• Dilution range: ELISA: 1:16000, WB: 0.1-0.3 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 55kDa and 40kDa bands seen in mouse brain extracts. Recommended for use at 0.5-2 μg/ml. Please note that the two bands may correspond to two different isoforms. Two isoforms of the epsilon mRNA have been detected in Northern blots (J Biol Chem 1996 Sep 6;271(36):22081-9).
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti PPP2R5E antibody, anti protein phosphatase 2,
• Note: For research use only
• Entrez: 5529

orb18322 staining (0.5ug/ml) of mouse brain extracts (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Western blot analysis of Human Cerebellum (Lane1), Mouse (Lane2) and Rat (Lane3) Brain lysate using PPP2R5E antibody