Goat anti-PPP2R3A Antibody

• Catalog Number: orb19698
• Category: Antibodies
• Description: Goat polyclonal antibody to PPP2R3A
• Target: PPP2R3A
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: QKDVENDGPEPSD
• RRID: AB_10748807
• MW: 130; 61.1
• Tested applications: ELISA, IHC
• Dilution range: ELISA: 1:16000, WB: 1 μg/ml, IHC-P: 5 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave an approx 80kDa band in Human Skeletal Muscle lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 130kDa according to NP_002709.2 and of 61.1kDa according to NP_871626.1. The 80kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti PPP2R3A antibody, anti protein phosphatase 2
• Note: For research use only
• Entrez: 5523

orb19698 (5µg/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.