Goat anti-PPP1R15A / GADD34 Antibody

• Catalog Number: orb18304
• Category: Antibodies
• Description: Goat polyclonal antibody to PPP1R15A
• Target: PPP1R15A / GADD34
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 2-4 μg/ml
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: AAALDLSGRRG
• RRID: AB_10750321
• MW: 73.4
• Tested applications: ELISA, FC, IF, IHC, WB
• Dilution range: ELISA: 1:128000, WB: 0.3-1 μg/ml, IHC-P: 2-4 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 75kDa band observed in lysates of cell line HEPG2 (calculated MW of 73.4kDa according to NP_055145.2). Recommended concentration: 0.1-0.3 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti PPP1R15A antibody, anti GADD34 antibody, anti
• Research Area: Apoptotic, Cancer, Epigenetics, Neuroscience, Sign
• Note: For research use only
• Entrez: 23645

Immunohistochemical staining of Human Liver using PPP1R15A antibody

Flow cytometric analysis of HepG2 Cells using PPP1R15A antibody

Western blot analysis of HepG2(lane 1), negative control KLY(lane 2) lysate using PPP1R15A antibody

Immunofluorescence analysis of HepG2 Cells using PPP1R15A antibody

Immunofluorescence analysis of HeLa Cells using PPP1R15A antibody