POU6F2 Antibody

SKU: orb20581

Description

Goat polyclonal antibody to POU6F2

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: ELISA: 1:16000, WB: 0.3-1 μg/ml
• Reactivity: Mouse
• Predicted Reactivity: Bovine, Human, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 65kDa band observed in Mouse Eye lysates (calculated MW of 73.3kDa according to Human NP_009183.3 and Mouse NP_778171.2). Recommended concentration: 0.3-1 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: POU6F2
• Molecular Weight: 73.3; 69.2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti POU class 6 homeobox 2 antibody, anti POU domain, class 6, transcription factor 2 antibody, anti retina-derived POU-domain factor-1 antibody, anti RPF-1 antibody, anti Wilms tumor suppressor locus antibody, anti WT5 antibody, anti WTSL antibody, anti POU6F2 antibody

orb20581 (0.3ug/ml) staining of Mouse Eye lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

POU6F2

NCBI Gene ID

11281