POU5F1B Antibody

SKU: orb20547

Description

Goat polyclonal antibody to POU5F1B

Images & Validation

• Tested Applications: ELISA
• Dilution Range: ELISA: 1:16000, WB: 1 μg/ml
• Predicted Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave an approx 28kDa band in Human Heart lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 38.6kDa according to NP_001153014.1. The 28kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: POU5F1B
• Protein Sequence: TPGYGSPHFTALYS
• Molecular Weight: 38.6
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti OCT4-pg1 antibody, anti octamer b antibody, anti OTF3C antibody, anti OTF3P1 antibody, anti POU 5 domain protein antibody, anti POU class 5 homeobox 1 pseudogene 1 antibody, anti POU class 5 homeobox 1B antibody, anti POU domain transcription factor Oct-4 antibody, anti POU domain, class 5, transcription factor 1 pseudogene 1 antibody, anti POU5F1P1 antibody, anti POU5FLC20 antibody, anti POU5FLC8 antibody, anti POU5F1B antibody

Quick Database Links

Gene Symbol

POU5F1B

NCBI Gene ID

5462, 5460