POU3F2/BRN2/OCT7 Antibody

SKU: orb20545

Description

Goat polyclonal antibody to POU3F2

Images & Validation

• Tested Applications: ELISA, WB
• Dilution range: ELISA: 1:8000, WB: 0.1-0.3 μg/ml
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Porcine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 45kDa band observed in Human Brain (Cerebellum) and in Mouse and Rat Brain lysates (calculated MW of 46.9kDa according to Human NP_005595.2 and 47.1kDa according to Mouse NP_032925.1). Recommended concentration: 0.1-0.3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: POU3F2 / BRN2 / OCT7
• Molecular Weight: 46.9
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Disclaimer: For research use only

Alternative Names

anti BRN2 antibody, anti OCT7 antibody, anti OTF7 antibody, anti POU class 3 homeobox 2 antibody, anti POU domain, class 3, transcription factor 2 antibody, anti POUF3 antibody, anti POU3F2 antibody

orb20545 (0.1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

POU3F2 / BRN2 / OCT7

NCBI Gene ID

5454