POU2F3 Antibody

SKU: orb20544

Description

Goat polyclonal antibody to POU2F3

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:32000, WB: 1-3 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 50kDa band observed in lysates of cell lines HEK293 and HepG2 (calculated MW of 47.4kDa according to NP_055167.2). Recommended concentration: 1-3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: POU2F3
• Protein Sequence: EPHLEASQHLPVPKH
• Molecular Weight: 47.4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti Epoc-1 antibody, anti FLJ40063 antibody, anti MGC126698 antibody, anti OCT11 antibody, anti PLA-1 antibody, anti POU class 2 homeobox 3 antibody, anti POU domain, class 2, transcription factor 3 antibody, anti POU transcription factor antibody, anti Skn-1a antibody, anti POU2F3 antibody

orb20544 (2ug/ml) staining of HEK293 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

POU2F3

NCBI Gene ID

25833