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POU2F1/OCT1 Antibody

SKU: orb125019

Description

Goat polyclonal antibody to POU2F1

Images & Validation

Tested ApplicationsELISA, WB
Dilution RangeELISA: 1:4000, WB: 0.5-2 μg/ml
ReactivityHuman
Predicted ReactivityBovine, Canine, Mouse, Porcine, Rat
Application Notes
WB: Approx 100kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 76.5kDa according to NP_002688.2). This molecular weight is routinely observed by other sources. Recommended concentration: 0.5-2µg/ml.

Key Properties

ClonalityPolyclonal
TargetPOU2F1 / OCT1
Protein SequenceNVQSKSNEESGDSQQ
Molecular Weight76.5
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti OCT1 antibody, anti Octamer-binding transcription factor-1 antibody, anti OTF1 antibody, anti OTTHUMP00000032348 antibody, anti POU class 2 homeobox 1 antibody, anti POU domain, class 2, transcription factor 1 antibody, anti POU2F1 antibody

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POU2F1/OCT1 Antibody

Western blot analysis of HeLa lysate using POU2F1 antibody

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

POU2F1/OCT1 Antibody (orb125019)

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Available Sizes

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100 μg
$ 560.00
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