Goat anti-POLL / POLkappa Antibody

• Catalog Number: orb18585
• Category: Antibodies
• Description: Goat polyclonal antibody to POLL
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Dilution range: ELISA: 1:32000, WB: 0.5-1 μg/ml
• Conjugation: Unconjugated
• MW: 63.5
• Target: POLL / POLkappa
• Entrez: 27343
• Protein Sequence: LGLPYREPAERDW
• RRID: AB_10748301
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti POLL antibody, anti BETA-N antibody, anti POL
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Small Intestine shows nuclear staining of enterocytes and of most cells in the lamina propria. Recommended concentration, 5-10ug/ml.WB: Preliminary experiments gave a band at approx 40kDa in Human Liver and HepG2 extracts at 1 μg/ml. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 66Kda according to NP_037406 (however please see comment above about Pol beta). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of Human Small Intestine using POLL antibody

Western blot analysis of A549 (Lane 1) and HepG2 (Lane 2) cell lysate using POLL antibody