Pnpla3/Adiponutrin (mouse) Antibody

SKU: orb19804

Description

Goat polyclonal antibody to PNPLA3

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:16000, WB: 0.01-0.03 μg/ml, IHC-P: 8 μg/ml
• Reactivity: Human, Mouse
• Predicted Reactivity: Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 45kDa band observed in Mouse Heart lysates (calculated MW of 42.6kDa according to Human NP_473429.2 and to Mouse NP_473429.2). Recommended concentration: 0.05-0.2 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Pnpla3 / Adiponutrin (mouse)
• Protein Sequence: EHDICPKVKSTN
• Molecular Weight: 42.6
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti Pnpla3 antibody, anti patatin-like phospholipase domain containing 3 antibody, anti Adpn antibody, anti adiponutrin antibody

orb19804 (0.03µg/ml) staining of Mouse Adrenal Gland lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

orb19804 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.

orb19804 (8µg/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

orb19804 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Mitochondrial staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). This data is from a previous batch, not on sale.

Quick Database Links

Gene Symbol

Pnpla3 / Adiponutrin (mouse)

NCBI Gene ID

80339