Plexin D1 Antibody

SKU: orb18973

Description

Goat polyclonal antibody to PLXND1

Research Area

Cardiovascular Disease

Images & Validation

• Tested Applications: ELISA, FC, IF
• Dilution Range: ELISA: 1:128000, IHC-P: 10ug/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Porcine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Preliminary experiments gave an approx 28kDa band in Human Bone Marrow lysates after 0.1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 212kDa according to NP_055918. The 28kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Plexin D1
• Protein Sequence: LAEPKKSHRQSH
• Molecular Weight: 212
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti PLXND1 antibody, anti PLEXD1 antibody, anti KIAA0620 antibody, anti plexin D1 antibody, anti MGC75353 antibody

Immunofluorescence analysis of HeLa cells using PLXND1 antibody

Immunofluorescence analysis of U251 cells using PLXND1 antibody

Flow cytometric analysis of K562 cells using PLXND1 antibody

Quick Database Links

Gene Symbol

Plexin D1

NCBI Gene ID

23129