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| Catalog Number | orb1550228 |
|---|---|
| Category | Antibodies |
| Description | PLCD3 (aa261-274) Antibody |
| Target | PLCD3 (aa261-274) |
| Species/Host | Goat |
| Conjugation | Unconjugated |
| Reactivity | Human |
| Predicted Reactivity | Bovine, Canine, Mouse, Porcine, Rat |
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Protein Sequence | HQYSGEDRVLSAPE |
| Tested applications | ELISA, IHC, WB |
| Dilution range | Peptide ELISA: antibody detection limit dilution 1:32000. |
| Application notes | WB: Approx 85kDa band observed in lysates of cell limes A549 and Caco-2, and in preliminary testing of cell lines HeLa, HEK293 and U251 lysate (calculated MW of 89.3kDa according to NP_588614.1). Recommended concentration: 1-3µg/ml. Primary incubation 1 hour . IHC: Paraffin embedded Human Kidney and Testis. Recommended concentration: 6µg/ml. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | PLCD3; phospholipase C, delta 3; MGC71172; 1-phosp Read more... |
| Note | For research use only |
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