Plakoglobin/Gamma-catenin Antibody

• Catalog Number: orb18674
• Category: Antibodies
• Description: Goat polyclonal antibody to JUP
• Target: Plakoglobin / Gamma-catenin
• Clonality: Polyclonal
• Species/Host: Goat
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Canine, Porcine
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Protein Sequence: GLRPPYPTADHMLA
• RRID: AB_10751429
• MW: 81.7
• Tested applications: ELISA, IHC, WB
• Dilution range: ELISA: 1:32000, WB: 0.1-0.3 μg/ml, IHC-P: 5 μg/ml
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 85kDa band observed in A431 lysate (predicted MW of 86kDa according to NP_002221, NP_068831). Recommended for use at 0.2-1 μg/ml.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti JUP antibody, anti GAMMA-CATENIN antibody, an
• Note: For research use only
• Entrez: 3728

orb18674 staining (0.2ug/ml) of A431 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Western blot analysis of A431 lysate (RIPA buffer, 35ug total protein per lane) using Plakoglobin antibody

Immunohistochemical staining of Human Heart using JUP antibody

Western blot analysis of A431 (Lane1) and NIH3T3 (Lane2) cell lysates using JUP antibody

Western blot analysis of Rat Eye lysate using JUP antibody