Plakoglobin/Gamma-catenin Antibody

SKU: orb18674

Description

Goat polyclonal antibody to JUP

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:32000, WB: 0.1-0.3 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Canine, Porcine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 85kDa band observed in A431 lysate (predicted MW of 86kDa according to NP_002221, NP_068831). Recommended for use at 0.2-1 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Plakoglobin / Gamma-catenin
• Protein Sequence: GLRPPYPTADHMLA
• Molecular Weight: 81.7
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti JUP antibody, anti GAMMA-CATENIN antibody, anti gamma-catenin antibody, anti junction plakoglobin antibody, anti DP3 antibody, anti DPIII antibody, anti ARVD12 antibody, anti CTNNG antibody, anti OTTHUMP00000164735 antibody, anti catenin (cadherin-associated protein), gamma (80kD) antibody, anti catenin (cadherin-associated protein), gamma 80kDa antibody

orb18674 staining (0.2ug/ml) of A431 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Western blot analysis of A431 lysate (RIPA buffer, 35ug total protein per lane) using Plakoglobin antibody

Immunohistochemical staining of Human Heart using JUP antibody

Western blot analysis of A431 (Lane1) and NIH3T3 (Lane2) cell lysates using JUP antibody

Western blot analysis of Rat Eye lysate using JUP antibody

Quick Database Links

Gene Symbol

Plakoglobin / Gamma-catenin

NCBI Gene ID

3728